ABSTRACT
Dihydrolipoamide dehydrogenase (LADH) from Trypanosoma cruzi, the causative agent of Chagas' disease, was inactivated by treatment with myeloperoxidase (MPO)-dependent systems. LADH lipoamide reductase and diaphorase activities decreased as a function of incubation time and composition of the MPO/H2O2/halide system, a transient increase preceding the loss of diaphorase activity. Iodide, bromide, thiocyanide and chloride were effective components of MPO/H2O2 or MPO/NADH systems. Catalase prevented LADH inactivation by the MPO/NADH/halide systems in agreement with H2O2 production by NADH-supplemented LADH. Thiol compounds (L-cysteine, N-acetylcysteine, penicillamine, N-(2-mercaptopropionylglycine) and Captopril prevented LADH inactivation by the MPO/H2O2/NaCl system and by NaOCl, thus supporting HOCl as agent of the MPO/H2O2/NaCl system. MPO/H2O2/NaNO2 and MPO/NADH/NaNO2 inactivated LADH, the reaction being prevented by MPO inhibitors and thiol compounds. T. cruzi LADH was affected by MPO-dependent systems like myocardial LADH, allowance being made for the variation of the diaphorase activity and the greater sensitivity of the T. cruzi enzyme to MPO/H2O2/halide systems.
Subject(s)
Animals , Humans , Hypochlorous Acid/pharmacology , Dihydrolipoamide Dehydrogenase , Neutrophils/physiology , Nitrites , Peroxidase , Protozoan Proteins/antagonists & inhibitors , Respiratory Burst , Trypanosoma cruzi , Acetylcysteine/pharmacology , Thioctic Acid/analogs & derivatives , Thioctic Acid/metabolism , Bromides , Captopril , Catalase , Cysteine/pharmacology , Sodium Chloride/pharmacology , Sodium Compounds/pharmacology , Cytotoxicity, Immunologic , Reactive Oxygen Species/metabolism , Glutathione , Glycine , Kinetics , Myocardium , NAD , Neutrophils/enzymology , Oxidation-Reduction , Penicillamine , Hydrogen Peroxide/pharmacology , Recombinant Proteins/antagonists & inhibitors , Sulfhydryl Compounds , Tryptophan , TyrosineABSTRACT
MDR1 promoter has been shown to contain heat shock elements (HSE), and it has been reported that FM3A/M and P388/M MDR cells show a constitutively activated heat shock factor (HSF), suggesting that HSF might be an important target for reversing the multidrug resistance. Therefore, it was examined whether quercetin, which has been shown to interfere with the formation of the complex between HSE and HSF, and to downregulate the level of HSF1, can sensitize MDR cells against anticancer drugs by inhibition of HSF DNA-binding activity. In this study, quercetin appeared to inhibit the constitutive HSF DNA-binding activity and the sodium arsenite-induced HSF DNA-binding activity in the MDR cells. The basal and sodium arsenite-induced MDRCAT activities were remarkably suppressed by the treatment of quercetin. These results were well consistent with the finding that the treatment of quercetin decreased the expression level of P-gp, MDR1 gene product, in dose-dependent manner, and markedly increased the sensitivity of MDR cells to vincristine or vinblastine. These results suggest that quercetin can decrease the expression of P-gp via inhibition of HSF DNA-binding activity, and might be useful as a chemosensitizer in MDR cells.
Subject(s)
Mice , Animals , Antineoplastic Agents/pharmacology , Arsenites/pharmacology , Carcinoma/drug therapy , Drug Resistance, Multiple/physiology , Drug Resistance, Neoplasm/physiology , Heat-Shock Proteins/metabolism , Heat-Shock Proteins/drug effects , Heat-Shock Proteins/antagonists & inhibitors , Leukemia, Experimental/drug therapy , ATP Binding Cassette Transporter, Subfamily B, Member 1/genetics , ATP Binding Cassette Transporter, Subfamily B, Member 1/drug effects , Quercetin/pharmacology , Sodium Compounds/pharmacology , Tumor Cells, Cultured , Vinblastine/pharmacology , Vincristine/pharmacologyABSTRACT
Intestinal uptake of lysine in rats progressively decreased with an increase in pH from 5.2 to 8.5, both in the presence and absence of Na+ ions. At pH 5.2 lysine uptake was 30-35% more than that at neutral pH. Na+ activated lysine uptake by 40-50% at pH 5.2 and it was increased to 110-120% at neutral pH. The observed increase in lysine uptake in response to Na+ and H+ gradients was due to enhanced maximal velocity (Vmax), with little change in affinity constant (Kt). Arrhenius analysis revealed a biphasic curve for lysine uptake with transition temperature (Tc) around 20 degrees C (24 degrees C at pH 5.2 in presence of Na+). The energy of activation (Ea) below (16.1-23.4 Kcal/mole) and above (6.7-8.6 Kcal/mole) the Tc was similar at pH 5.2 and 7.0 both in the presence and absence of Na+ ions. The sensitivity of lysine uptake to various inhibitors was also dependent upon pH and Na+ ions.